To find out new determinants required for Nef activity we performed a functional alanine scanning analysis along a discrete but highly conserved region at the core of HIV-1 Nef. We identified the GPG-motif. located at the 121-137 region of HIV-1 NL4. 3 Nef. https://www.diegojavierfares.com/amazing-price-Kicker-48CDF104-Comp-10-Down-Firing-Loaded-Subwoofer-Enclosure-special-find/